WebSep 6, 2024 · CYTOO is a preclinical and clinical-stage drug discovery biotech company focusing on disorders affecting muscle health. CYTOO has pioneered the ability to control cells microenvironment and has developed a platform of micropattern technologies to enable significantly greater physiologically relevant cellular models in comparison to standard ... WebDec 11, 2024 · work on the CYTOO chips overseeing research assistants Saranya Santoosh Kumar and Carolyn Park. Competing Interest Statement: No competing interests Classification: Major Biological Sciences available under …
(PDF) Mapping cell migrations and fates from a ... - ResearchGate
WebJun 8, 2024 · The side of the CYTOO chip on which the CYTOO label is written in the forward direction is the patterned surface. If necessary gently press the borders of the chip down with the tweezers to ensure that the entire surface of the chip is wetted. 4. Incubate the chip at 37 °C for 3 h or overnight at 4 °C. 5. Wash the chip three times with PBS++. diameter of mesenteric arteries
Cytoo, Buoyed by Nature Methods Study, to Debut New …
WebJul 25, 2024 · Seeding of the cells in CYTOO chips is a crucial step for obtaining enough cells with the desired cellular architecture for imaging. Seeding too many cells or extremely mild washing leads to multiple cells attaching to a single pattern eventually inhibiting the spreading of the cytoplasm of a single cell on the micropattern. On the other hand ... WebAug 9, 2024 · Cells were then spread onto fibronectin Cytoo chips (Cytoo Cell Architect) 4 to 5 hr before imaging. Cell adhesion on micro-patterns both constrains the cells in terms of lateral movement and averages their size and shape (disk-shaped and crossbow-shaped, Cytoo Cell Architect, 1100 μ m 2). As a control of patterning effect, the same cell line ... WebSep 12, 2024 · Once photo-converted, each CYTOO chip was returned to its native 35 mm dish and placed in an incubator. For tracking these cells at later times, the micropatterned chips were taken out of the incubator and sequentially re-loaded in the CYTOO holder and imaged with the Leica SP8 confocal microscope (see Imaging and Image Analysis section). diameter of mega roll of toilet paper