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Web2. Incubate membrane in Ponceau S Staining Solution for 5-10 minutes at room temperature. 3. Wash membrane in ddH 2 O until distinct reddish-pink protein bands are visible (1-5 min). 4. If desired, mark proteins with … WebSpot Treat You can wash wool sweaters, jackets, blankets, winter accessories, and more from home, but if your wool item is structured or has a lining as a blazer, coat, or suit typically would, spot treat stains and odors instead of giving it a full wash. To spot treat stains and odors on your woolen, use our Stain Solution and gently work into affected … Ponceau S, Acid Red 112, or C.I. 27195 (systematic name: 3-hydroxy-4-(2-sulfo-4-[4-sulfophenylazo]phenylazo)-2,7-naphthalenedisulfonic acid sodium salt ) is a sodium salt of a diazo dye of a light red color, that may be used to prepare a stain for rapid reversible detection of protein bands on nitrocellulose or polyvinylidene fluoride (PVDF) membranes (western blotting), as well as on cellulose acetate membranes. … dyson v10 motorhead hepa filter